Cross-linking of nucleosomal histones with monofunctional imidoesters.
نویسندگان
چکیده
Cross-linking experiments with the MONOfunctional imidoester methyl-acetimidate, in the pH range 7.0 - 8.0, on rat liver nucleosomes generate a cross-linking pattern almost identical with the one observed for much longer BIfunctional reagents (e.g. dimethylsuberimidate). Combined cross-linking and trypsin digestion experiments suggest that all or at least the great majority of this cross-linking occurs on trypsin digestible segments (or "tails") of the histones. The formation of oligomers over such extremely short distances and especially the observation of an H3 homodimer suggests a very close proximity of half-nucleosomes.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 5 7 شماره
صفحات -
تاریخ انتشار 1978